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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: Appl Opt. 2008 Nov 1;47(31):5944–5952. doi: 10.1364/ao.47.005944

Fig. 3.

Fig. 3

IRDye800CW does not affect proliferation and function of EBV-specific CTLs. (a) IRDye800CW-labeled and nonlabeled EBV-specific CTLs were stimulated with autologous LCL enumerated for proliferation. No difference in CTL expansion was observed over a 14 day period. (b) IRDye800CW-labeled and nonlabeled CTLs were analyzed for the effect of IRDye800CW binding on cytotoxic function against autologous and allogeneic LCL. Both nonlabeled and IRDye800CW-labeled CTLs were capable of recognizing and lysing autologous LCL but not allogeneic LCL. (c) Labeled and unlabeled control CTLs were compared for IFN-γ production in an ELIspot assay against autologous and allogeneic LCL. IRDye800CW did not affect the ability of CTLs to produce IFN-γ.