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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: Appl Opt. 2008 Nov 1;47(31):5944–5952. doi: 10.1364/ao.47.005944

Fig. 8.

Fig. 8

In vivo migration assays demonstrate specific NIR signal accumulation by CCL5-homing CTLs. (a) EBV-specific CTLs and OKT3 stimulated T cells express the chemokine CCR5 and migrate in response to CCL5 chemokine secretion. Here, EBV-specific CTLs show specific migration towards CCL5 in transwell migration assays. (b) To demonstrate specific NIR signal accumulation by CTLs, nonmodified Karpas tumor cells (K-NT) or Karpas cells transduced with the chemokine CCL5 (K-CCL5) were injected s.c. into the left and right flank, respectively. Next, IRDye800CW-labeled CTLs were injected i.v., and mice were imaged for NIR signal after 48 h. NIR signal accumulated in K-CCL5 tumors but not in K-NT tumors, demonstrating that NIR signal is dependent on migration of CTLs into the tumor tissue rather than any nonspecific accumulation. (c) Both K-NT and K-CCL5 tumors and local inguinal lymph nodes (LN) were excised and imaged for NIR signal. As observed by noninvasive imaging, K-CCL5 showed higher NIR signal than K-NT tumors, indicating that CTL migration was responsible for NIR signal accumulation.