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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Protein Expr Purif. 2008 Sep 26;63(2):128–133. doi: 10.1016/j.pep.2008.09.012

Figure 2. Chromatogram showing the Ni-NTA purification of the protein eluted from the PEI pellet.

Figure 2

The absorbance at 280 nm is shown as a solid black line. The absorbance at 260 nm is shown as a dashed black line. The concentration gradient of Buffer B is shown as the slanted black line. EBNA1 eluted at approximately 215 mM imidazole. Fractions C5–C10 were pooled.