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. Author manuscript; available in PMC: 2010 Feb 1.

Published in final edited form as: Protein Expr Purif. 2008 Sep 26;63(2):128–133. doi: 10.1016/j.pep.2008.09.012

A) SDS-PAGE showing the PEI fractionation, ammonium sulfate (AS) precipitation, and Ni-NTA chromatography steps. FT is flowthrough. Gel is stained with Coomassie. B) Western blot probed with mAbs 1EB14 and 3EB7 shows that EBNA1 is not lost in the 0.1 M and 0.3 M NaCl wash steps. Some EBNA1 remains trapped in the PEI pellet and is released in a second high salt wash (TE + 1 M NaCl), and no EBNA1 is lost during the AS precipitation. PEI washes are listed with the NaCl concentration used. MW is molecular weight markers.

A) SDS-PAGE showing the PEI fractionation, ammonium sulfate (AS) precipitation, and Ni-NTA chromatography steps. FT is flowthrough. Gel is stained with Coomassie. B) Western blot probed with mAbs 1EB14 and 3EB7 shows that EBNA1 is not lost in the 0.1 M and 0.3 M NaCl wash steps. Some EBNA1 remains trapped in the PEI pellet and is released in a second high salt wash (TE + 1 M NaCl), and no EBNA1 is lost during the AS precipitation. PEI washes are listed with the NaCl concentration used. MW is molecular weight markers.