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. Author manuscript; available in PMC: 2010 Feb 1.

Published in final edited form as: Protein Expr Purif. 2008 Sep 26;63(2):128–133. doi: 10.1016/j.pep.2008.09.012

A) The chromatogram resulting from further purification of the Ni-NTA eluate over a MonoS column. Absorbance at 280 nm is shown as a solid black line. Absorbance at 260 nm is shown as a dashed black line. The salt gradient utilized is shown as the slanted black line. B) SDS-PAGE (Coomassie stained) showing EBNA1 purification by MonoS chromatography. FT is flowthrough. MW is molecular weight markers.

A) The chromatogram resulting from further purification of the Ni-NTA eluate over a MonoS column. Absorbance at 280 nm is shown as a solid black line. Absorbance at 260 nm is shown as a dashed black line. The salt gradient utilized is shown as the slanted black line. B) SDS-PAGE (Coomassie stained) showing EBNA1 purification by MonoS chromatography. FT is flowthrough. MW is molecular weight markers.