FIG. 6.

RA increases type X collagen in scaffolds seeded with CP chondrocytes. Immunohistochemical analyses of type X collagen was performed on deparaffinized sections of CP (A–D) and CD (E–H) chondrocytes. Sections were counterstained with alcian blue to visualize proteoglycans and light green to label the chitosan walls. Photomicrographs (A), (B), (E), and (F) correspond to 10 days in culture, and pictures (C), (D), (G), and (H) correspond to 20 days in culture. Upper panels show controls (incubated with preimmune serum) of respective bottom panels. Color images are available online at www.liebertonline.com/ten.