Figure 2.

Fluorescence decays and multi-exponential fits of the dark adapted Y21F mutant and AppA wt. Right image: AppAwt was illuminated with strong blue light for 2 minutes and data collection was done for 1 minute immediately after illumination. Red line represents instrument response function. Results of multiexponential deconvolution can be found at Table 1. The values of χ² are following: 1.29 for Y21F (left), 1.32 for AppAwt dark form (middle), and 0.99 for AppAwt light form (right).