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a) Histogram showing the number of identified peptides and protein comparing 3 versus 5 min reduction and alkylation times and 1, 2 and 3 min digestion times using ultrasound irradiation. b-c) Histograms showing the protein coverage (b) and number of unique peptides (c) for 4 different proteins during five technical replicates after 3 min reduction and alkylation time and 1 min digestion time using ultrasound irradiation.
