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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2008 Nov 14;50(4):1791–1800. doi: 10.1167/iovs.08-2487

Figure 10.

Figure 10

The effect of DIDS and acetazolamide on baseline cytoplasmic pH of porcine cultured NPE. BCECF-loaded cells were first superfused with bicarbonate-containing buffer for 3 min to establish baseline cytoplasmic pH. At this point DIDS 100 µ, acetazolamide 500 µM or vehicle DMSO 0.1% was introduced (arrow) and data collection was continued for a further 20 min. The results are shown as mean±SEM of 7–10 independent experiments. At the final time point, pHi in DIDS-treated cells showed a significant difference from control ***P <0.001.