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The effect of DIDS (100 µM), low chloride buffer, CA inhibitors acetazolamide (500 µM) and methazolamide (100 and 500µM) and sodium-hydrogen exchange inhibitor DMA (100 µM) on the rate of gradual acidification towards baseline following the rapid pHi rise caused by the removal of HCO3−/CO2 and replacement with HEPES buffer. The results are the mean±SEM of data from 6 or 10 independent experiments. A significant difference from control is indicated by ***P <0.0001.
