Figure 2. α1-containing GABA_ARs are redistributed in PCs of ax ^J mice.

GABA_AR α1 staining of PCs in slices of wt or ax ^J mice using Cy3- (A) or biotin- (B) labeled secondary antibodies. Scale bars: 20 µm. (C) EM analysis of GABA_AR α1 using biotin-coupled secondary antibodies. Scale bars: 500 nm. (D) Western blot analysis of protein extracts from wt or ax ^J cerebella reveals that the effects seen in (A–C) represent a receptor redistribution rather than a change in total gene expression. Actin serves as a loading control. (E) In situ hybridisation using GABA_AR α1 oligonucleotides revealed comparable mRNA levels in PC somata (arrows) as well as in dendrites of wt and ax ^J mice shown in dark (upper panels, white dots) and bright (lower panels, blue dots) field images. Nuclei were visualized by Hämalaun staining. Note that GABA_AR α1 mRNA signals were absent upon use of sense control oligonucleotides (right panels). Scale bar, upper panel: 100 µm. Scale bar, lower panel: 50 µm. gl: granular layer; ml: molecular layer; pc: Purkinje cell layer.