Figure 7. Representative images and quantification of CD11b and VEGFR1 positive cells at the periphery of lung metastases.

(A–F) Confocal microscopy images of lung tissue from C57BL mice (WT, A–C) and flt-1 ^TK–/–/C57BL (KO, D–F) mice after immunostaining for CD11b (using FITC-labeled anti-CD11b antibody, in green in B and E), or for VEGFR1 (using AF647-labeled MF1 antibody, in red in C and F). Sections were counterstained with DAPI nuclear dye (in blue in A–F). The number of positive cells was calculated as the ratio of CD11b⁺ or VEGFR1⁺ surface area to DAPI surface area (G). A statistically significant difference (p<0.05 by Student's t-test) is identified with an asterisk. All images are 512 µm across.