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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: Nat Methods. 2007 Mar 18;4(4):319–321. doi: 10.1038/NMETH1024

Figure 1.

Figure 1

Bleaching steps of single fluorescent protein complexes reveal the number of GFP-labeled subunits. (a) A single image of the acquired sequence shows the selected fluorescent spots in the cell membrane (blue circles). Scale bar, 2 μm. (b) The different labeling schemes include 1, 2 or 4 subunits of a membrane protein complex fused to GFP. (c) Time courses of fluorescence emission for the labeling schemes in b (two examples each from spots bleaching with the expected number of bleaching steps). Green arrows indicate the bleaching steps. The y-axis is scaled in photons per second for comparison purposes (for calibration see Supplementary Methods).