Figure 3.

Calcitonin gene related peptide (CGRP) was measured from the lumbar spinal cord tissue of ICR, CB₁WT, and CB₁KO mice. After a 45 min equilibration period in the perfusion chambers, followed by a 12 min collection of perfusate for determination of baseline values, capsaicin (1 μm) was added to the perfusion medium. CGRP content was quantified by radioimmunoassay. Evoked release was defined as the amount of CGRP above the basal values. Capsaicin-evoked CGRP release from spinal cord tissue of ICR and CB₁WT mice was significantly higher when compared with spinal cord tissue from CB₁KO mice (*p < 0.05, n = 6 in all groups).