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. Author manuscript; available in PMC: 2010 May 26.
Published in final edited form as: Biochemistry. 2009 May 26;48(20):4285–4293. doi: 10.1021/bi900151g

Figure 6. V477D Mutant is Deficient in β2AR-mediated Activation.

Figure 6

Phosphorylation of 1 mM RASASA peptide by 100–200 nM GRK2 or 200 nM V477D was assessed in the absence or presence of PI/DDM mixed micelles (PI/DDM), 80 nM isoproterenol-treated β2AR in PI/DDM mixed micelles (β2AR/ISO), or 80 nM alprenolol-treated β2AR in PI/DDM mixed micelles (β2AR/ALP). Two-way ANOVA was used to test statistical significance. Error bars indicate SEM. *, p<0.05; **, p<0.01; ***, p<0.001.