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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: J Cereb Blood Flow Metab. 2009 May 6;29(7):1229–1239. doi: 10.1038/jcbfm.2009.48

Figure 5. Confirmation of ED-2:PB Double-staining.

Figure 5

ED-2+ and PB+ macrophages were identified (top, middle row), validating the presence of iron in well-differentiated macrophages. ED-1+ and PB− macrophages were identified (bottom row), confirming the absence of iron in monocytes/macrophages, and activated microglia. Brown = ED-2+ (top, middle) or ED-1+ (bottom), Blue = PB+, Background = nuclear fast red counterstain. Colocalized images, 40×, scale bar = 25 µm.