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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: J Immunother. 2008 May;31(4):359–369. doi: 10.1097/CJI.0b013e318165f5d2

Figure 4.

Figure 4

DCs transduced with Ad5f35-null vector and matured using the standard cocktail generate the most adenovirus antigen-specific cells. DCs transduced with Ad5f35 were matured using the 3 prototype maturation cocktails: (i) TNF-α, PGE2; (ii) IL-1, IL-6, TNF-α, and PGE2; and (iii) IL-1, IL-6, TNF-α, IFN-α, and CD40L were used to stimulate autologous responder T cells at a stimulator to responder ratio of 1:20. Proliferation of antigen-specific T cells was determined after 9 days. A, The frequency of CD8+ TDL and TYF-specific cells was determined using pentamer staining. The results are expressed as percent CD8+ pentamer+ cells. B, Absolute numbers of TDL and TYF specific cells were determined using the absolute CTL proliferation and pentamer percentages. C, IFN-γ production in response to irradiated Ad5f35-transduced stimulators using an IFN-γ ELISPOT assay is presented as SFC/1 × 105 CTL (subtracted from control). The results shown are for donor 3.