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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: Cancer Res. 2008 Oct 1;68(19):8094–8103. doi: 10.1158/0008-5472.CAN-07-6208

Figure 2.

Figure 2

Expression analysis and methylation analysis of CD133 gene in colon cancer and glioblastoma(GBM) cell lines. (A, C) CD133 expression levels were examined in (A) twelve colorectal cancer cell lines (HCT116, DKO, SW480, RKO, HT29, Caco-2, COLO 320, Lovo, COLO 205, DLD1, SW48, and SW620) and normal colon (NC) and (C) 6 GBM cell lines (T98G, U87 MG, D566 MG, D263 MG, D54 MG, and HSR-GBM1) and normal brain (NB). Lanes marked + are with and those −, without treatment with DAC (5-aza-2′-deoxycytidine). The gene Actin B serves as a positive control for RNA quality and loading in both A and C. ddH2O=water control adding no cDNA. (B, D) MSP analysis of the DNA methylation status of the 5′ CpG island in the CD133 gene in a panel of (B) colon cancer and (D) Glioblastoma (GBM) cell lines. M=methylation signal; U=unmethylated signal. IVD= in vitro methylated DNA. NC and NB indicate normal colon tissue and normal brain tissue, respectively. ddH2O=water control adding no DNA.