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. Author manuscript; available in PMC: 2010 Feb 5.
Published in final edited form as: Vaccine. 2008 Dec 11;27(6):870–881. doi: 10.1016/j.vaccine.2008.11.084

Figure 1.

Figure 1

(A) Sequential A3G mRNA expression in PBMC before and after (x3) immunization (●) n=15 and unimmunized control (o) n=5 rhesus macaques, which were challenged with SIVmac251 (at week 27, 4 weeks after the 3rd immunization). Blood was collected before each of the 3 immunizations and SIV challenge. The results are presented as mean ± sem; ≤0.02*, <0.01**, <0.005***, <0.0002****. (B) Western blots before and after the 3rd immunization (week 27) with lysates of PBMC from 4 macaques. (C) Intracellular expression of A3G proteins in CD4+ T cells detected by flow cytometry before and after 3rd immunization (week 27). (D) A3G mRNA and protein knock down with A3G specific and non-specific siRNA of CD4+ T cells from 4 animals and representative flow cytometry.