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. Author manuscript; available in PMC: 2010 Jul 10.
Published in final edited form as: Mol Cell. 2009 Jul 10;35(1):93–104. doi: 10.1016/j.molcel.2009.05.012

Figure 5. Mutational analysis of the Cdt1 PIP box.

Figure 5

(A) HSS was mixed with MMS plasmid and 50 nM recombinant Cdt11-243, Cdt11-243/ΔPIP, Cdt11-243/T5A, or Cdt11-243/K+4A. Reactions were stopped at different times and blotted for Cdt1 or Cdt11-243-GST (anti-GST antibody).

(B) HSS was mixed with MMS plasmid and 50 nM recombinant Cdt11-243, Cdt11-243/ΔPIP, or Cdt11-243/D6A. Reactions were stopped at different times and blotted as in (A).

(C) HSS was mixed with MMS plasmid and 50 nM recombinant Cdt11-243, Cdt11-243/ΔPIP, Cdt11-243/R+5A or Cdt11-243/T5D. Reactions were stopped at the indicated times and blotted as in (A).