Table III.

miRNAs differentially expressed by microarray and qRT–PCR and their mRNA targets (P < 0.05)

Down-regulated miRNA	miRNA Ct valuea	mRNA targets		Target mRNA gene ID	Gene name	Fold change
		Up	Down
miR-9	30.29 ± 0.38 versus 26.94 ± 1.04	31	63	CALB2	Calbindin 2	+3.77
				CCNE2	Cyclin E2	+3.29
				TGFBI	TGF, β induced	+3.14
				FBN1	Fibrillin 1	+2.03
				BCL2	B cell CLL/lympoma 2	+1.72
				FGFR2	Fibroblast growth factor receptor 2	+1.65
miR-34c-5p	29.09 ± 0.57 versus 27.88 ± 0.46	18	20	CCNE2	Cyclin E2	+3.29
				BCL2	B cell CLL/lympoma 2	+1.72
				CDK6	Cyclin-dependent kinase 6	+1.51
miR-34b*	26.22 ± 0.29 versus 24.21 ± 0.36	10	14	MAPK4	Mitogen-activated protein kinase 4	+1.98
				BRD4	Bromodomain containing 4	+2.20
				CDK6	Cyclin-dependent kinase 6	+1.51

These were identified by cross-referencing the predicted mRNA gene targets of each miRNA against the gene transcripts that were found to be differentially expressed in endometrium from women with versus without moderate–severe endometriosis in previous global gene expression analysis (Burney et al., 2007).

^aMiRNA Ct value from RT–PCR validations expressed as mean ± SEM in endometriosis versus nonendometriosis specimens.