Splenocytes and thymocytes were isolated from three XIAP WT and three XIAP KO mice, and stained with anti-CD24, NK1.1+ and TCR-β+ (A and B), as well as PE-conjugated α-galactosylceramide-loaded CD1d tetramer, specific for NKT cells (C and D). NKT cells are defined as CD24low, NK1.1+, TCR-β+, and tetramer+. A and C show total results of at least 3 individual mice, error bars shown are standard error of the mean. B and D are representative FACS plots of the CD24low subpopulation.