Table 1. Lymphocyte subsets based on cell surface markers as determined by four-color flow cytometry.
Cell Marker | ||||
T cell subtype | FITC | PE | APC | PerCP |
CD4+ T cells | CD3+ | CD20− | CD4+ | CD8− |
CD8+ T cells | CD3+ | CD20− | CD4− | CD8+ |
B cells | CD3− | CD20+ | ||
central memory CD4+ T cells | CD95+ | CD28+ | CD3+ | CD8− |
effector memory CD4+ T cells | CD95+ | CD28− | CD3+ | CD8− |
naïve CD4+ T cells | CD95− | CD29+ | CD3+ | CD8− |
CCR5+ memory CD4+ T cells | CD45RA− | CCR5+ | CD3+ | CD8− |