Table 1.

AAV-1 peptide epitopes identified

Peptides identified by IFN-γ ELISpot	Amino acid position	Peptide library pools	Peptide library no.	HLA binding prediction	MHC restriction
Subject A
SSFYCLEYFPSQMLRTGNNF	390	7-19/20	79/80	N/D	II†
Subject D
TTSTRTWALPTYNNH	247	5-13	49	HLA-DR*0801	II†
TTSTRTWALPTYNNH	243	5-13	49	HLA-DR*1302	II†
Subject E
TTSTRTWALPTYNNH	240	5-13	49	N/D	II†
YTEPRPIGTRYLTRP	724	12-13	145	HLA-B*1501	I‡
EFSATKFASFITQYS	665	12-13	133	HLA-DR*1501	II
Subject H
VQVFSDSEYQLPYV	341	6-21	69	HLA-A*3301	I

Bold, underlined amino acid sequences represent the epitopes predicted using online MHC-binding prediction programs.^19,20 Amino acid position indicates the position of the first amino acid of the epitope in the AAV-1 VP1 capsid protein sequence. MHC restriction indicates whether CD8⁺ (class I) or CD4⁺ (class II) T cells recognize the epitopes identified.

N/D indicates not determined.

^†Confirmed by IFN-γ ELISpot assay.

^‡Confirmed by IFN-γ intracellular staining.