Figure 5.

EM analysis of normal human cortex under the standard protocol used for mice (Stoppini et al. 1991; Tanaka et al. 1994). A, 7 days in culture. B, 14 days in culture. Under these conditions, the cytoarchitecture of the explants were not preserved even at 7 days. With continued culturing, less cell bodies were observed, membranes appeared more diffuse and irregular, and synaptic contacts and vesicles were less infrequently observed as compared with the revised protocol.