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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Nat Struct Mol Biol. 2009 Jul 20;16(8):883–889. doi: 10.1038/nsmb.1637

Figure 1.

Figure 1

GST-Ede1 UBA is selective for K63-linked polyUb. SPR analysis of GST-Ede1 binding to K63- and K48-linked (a) Ub2 and (b) Ub4 reveals preference for K63-linked polyUb (upper panels). The residuals (lower panels) indicate a systematic deviation from the 1:1 binding model used to fit the K63-linked polyUb data. These data are the averages of three or four independent experiments, except K48-Ub4, which is the average of two trials. (c) GST-Ede1 was used to pull-down radiolabeled Ub4 chains (upper panel). The lower panel shows even loading of GST-Ede1 or control GST onto the glutathione beads. The averages of three such experiments are quantified in (d). The error bars indicate standard deviations for the three trials.