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Dissociation equilibrium constants (Kd) for GST-UBA domains interacting with polyubiquitins.
| GST-UBA | K63-Ub2 | K48-Ub2 | K63-Ub4 | K48-Ub4 |
|---|---|---|---|---|
| Ede1 | 48 ± 9 | 110 ± 16 | 6.0 ± 1.9 | 25 |
| hHR23A UBA1 | 26 | 36 | 4.7 | 13 |
| Ubc1 | 130 | 460 | ND | ND |
| DRM2 UBA1 | 89 | 140 | ND | ND |
Kd values (μM) were determined by SPR. Measurements with 3 or 4 replicates are presented as (Kd ± standard deviation). ND, not determined.
