Table 2.
Dissociation equilibrium constants (Kd) for fluorescent UBA domains interacting with polyubiquitins.
UBA | K63- Ub2 | K48- Ub2 | K63- Ub4 | K48- Ub4 |
---|---|---|---|---|
Ede1 | 120 | 86 | 54 | 31 |
hHR23A UBA1 | ND | ND | 30 | 9.0 |
Ubc1 | ND | ND | 170 | 37 |
Dsk2 | ND | ND | 2.2 | 1.4 |
Ddi1 | ND | ND | 92 | 50 |
Kd values (μM) were determined by fluorescence anisotropy. ND, not determined.