. Author manuscript; available in PMC: 2010 Feb 1.

Published in final edited form as: Nat Struct Mol Biol. 2009 Jul 20;16(8):883–889. doi: 10.1038/nsmb.1637

Table 2.

Dissociation equilibrium constants (K_d) for fluorescent UBA domains interacting with polyubiquitins.

UBA	K63- Ub₂	K48- Ub₂	K63- Ub₄	K48- Ub₄
Ede1	120	86	54	31
hHR23A UBA1	ND	ND	30	9.0
Ubc1	ND	ND	170	37
Dsk2	ND	ND	2.2	1.4
Ddi1	ND	ND	92	50

K_d values (μM) were determined by fluorescence anisotropy. ND, not determined.