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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Int J Parasitol. 2009 May 20;39(13):1425–1439. doi: 10.1016/j.ijpara.2009.05.001

Fig. 3.

Fig. 3

Regulatory elements from the Plasmodium falciparum Group A var gene PFD1235w repress cumulative luciferase activity from the UpsA promoter and affect the timing of maximal transcription. (A) Histogram of the percentages of each developmental stage obtained at the harvested time points. Representative Giemsa-stained images are presented, with the border color corresponding to the bar color on the histogram. Parasite lines were subjected to repeat rounds of double sorbitol synchronization, with the initial round performed just after blood cell invasion and a second one performed 12-15 h later. Replicate flasks were prepared to minimize parasite culture handling. (B) relative luciferase units (RLU) per million parasites (RPM) obtained from the time course experiment. (C) From this experiment, the cumulative area under the curve values were derived using a trapezoidal formula.