Table 2.
Means of log-transformed relative luciferase units per million Plasmodium falciparum parasites (log(RPM)) and related statistics.
| Parasite line | Mean of log(RPM)a | Difference of the mean log(RPM) valuesb | 95% C.I. of Differencec | Reciprocal ratio of expressiond | Reciprocal 95% C.I. of Ratiod |
|---|---|---|---|---|---|
| ALH | 0.314 | 0 | n/a | 1 | n/a |
| ALHi | -0.353 | 0.667 | 0.0315, 1.303 | 0.215 | 0.050, 0.93 |
| ALA1 | -0.884 | 1.198 | 0.563, 1.834 | 0.063 | 0.015, 0.27 |
| ALA2 | -0.775 | 1.089 | 0.454, 1.725 | 0.081 | 0.019, 0.35 |
| ∂LH | -4.426 | 4.633 | 1.750, 7.517 | 0.00002 | 3 × 10-8, 0.018 |
| HLH | 3.692 | 0 | n/a | 1 | n/a |
| HLHi | 3.601 | 0.090 | -0.410, 0.591 | 0.813 | 0.26, 2.56 |
| HLA | 2.904 | 0.787 | 0.287, 1.288 | 0.163 | 0.051, 0.51 |
Log10 transformation of the RPM values corrects for non-Gaussian distributions and permits evaluation of the fold-difference in expression.
Values were calculated by subtracting the mean of the log-transformed RPM values of each parasite line from the control line mean value. Mean ALHi, ALA, and ∂LH log(RPM) values were subtracted from the mean ALH log(RPM). Mean HLHi and HLA log(RPM) values were subtracted from the mean HLH log(RPM).
The 95% confidence interval (C.I.) of the difference in mean log(RPM) values were obtained from repeated measures ANOVA with Dunnett’s post tests performed separately on the UpsA-and the hrp3-promoter containing lines. ALH and HLH were defined as control lines for the respective groups. The 95% C.I. of the ∂LH line was obtained by a paired t test comparison of log-transformed ∂LH values with the matched log-transformed ALH values.
The ratio (or fold difference) of luciferase expression was the antilog of the difference in mean log(RPM). This was calculated per the formula: log(control) - log(treated) = log (control/treated). The reciprocal of the antilog was presented to show expression as the fold reduction from ALH or HLH.
The 95% C.I. of the ratio of expression versus ALH or HLH was calculated from the antilog of the 95% C.I. of the difference in the mean log(RPM) values. The reciprocal value was presented to illustrate the fold reduction in expression. n/a, not applicable.