Fig. 2.
A: Gelatin zymography of MMP-2 and MMP-9 in uterine homogenates from Siberian hamsters (n = 10/group) exposed to short days (8L: 16D) or LD (control) for 3, 6, 9, or 12 weeks. Columns with different letters differ significantly (P < 0.05). Active MMP-9 (80–82 kDa); proMMP-2 (72 kDa) and active MMP-2 (60 kDa) bands are labeled. B: Gelatin zymography of MMP-2 and MMP-9 in uterine homogenates from Siberian hamsters (n = 10/group) exposed to either SD or LD (controls) for 14 weeks then SD exposed animals were subsequently transferred to LD for 0, 1, 2, 4, or 8 weeks. Columns with different letters differ significantly (P < 0.05). C: Zymograms were also developed in the presence of: no inhibitors, MMP-1 inhibitor, serine protease inhibitor, 5 mM EDTA or GM6001 as described under methods.
