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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Jul 14;1793(10):1604–1613. doi: 10.1016/j.bbamcr.2009.07.001

Fig. 4. NRF-1 silencing suppresses Nos1 and COX subunit mRNAs.

Fig. 4

(A) Western blot reveals a down-regulation of NRF-1 and NOS1 protein levels in NRF-1 shRNA-transfected neuron, whereas NOS2 protein levels were not affected. β-Actin served as a loading control. (B–C) N2a cells and primary neurons were transfected with shRNA against NRF-1 (light gray bars), or with empty vectors (black bars), or with scrambled shRNA (dark gray bars). NRF-2α served as a negative control. NRF-1, Nos1, COX2, and COX6c subunit mRNAs show significant decreases in shRNA-treated samples as compared to those with empty vectors, whereas Nos2, Nos3, and NRF-2α mRNA remained unchanged. N = 6 for each data point. *, P < 0.05; **, P < 0.01 as compared to empty vectors.