Table 2.

Table 2A EMSA Probes
Positions of probes are given relative to TSP. Putative NRF-1 binding sites are in boldface. Mutated nucleotide sequences are underlined.
Gene Promoter	Position	Sequence
Nos1	+150/+164	F 5′TTTTGTGCCGCGGAGCAGAGCGGCCTT 3′ R 3′  CACGGCGCCTCGTCTCGCCGGAATTTT 5′
Nos2	−98/−73	F 5′TTTTAGTTATGCAAAATAGCTCTGCAGAG 3′ R 3′  TCAATACGTTTTATCGAGACGTCTCTTTT 5′
Nos3	−114/−89	F 5′TTTTCCACATTAAATACGCAACAAATAGA 3′ R 3′  GGTGTAATTTATGCGTTGTTTATCTTTTT 5′
Nos1 with mutated NRF-1 site	−49/−24	F 5′TTTTGTAAAGCGGAAAAGAGCGGCCTT 3′ R 3′  CATTTCGCCTTTTCTCGCCGGAATTTT 5′
Rat Cyt C	−172/−147	F 5′TTTTCTGCTAGCCCGCATGCGCGCGCACCTTA3′ R 3′  GACGATCGGGCGTACGCGCGCGTGGAATTTTT5′

Table 2B Chip Primers
Positions of amplicons are given relative to TSP.
Gene Promoter	Position	Sequence	Amplicon length
Nos1	−134 to +68	F 5′AAACGCAAAGTGGGAGGTCT 3′ R 5′GCATGGCTGGTTTACGTTTT 3′	202 bps
Nos2	−140 to +5	F 5′AGCTAACTTGCACACCCAACT 3′ R 5′GGGCCAGAGTCTCAGTCTTC 3′	145 bps
Nos3	−171 to +17	F 5′GGTATTTGATGCTCGGGACT 3′ R 5′CACTGTGATGGCTGAACTGA 3′	188 bps
TFB2M promoter	−64 to +115	F 5'GAAGCGAGTGAGCAAAGGAC 3' R 5'GGTCCCCTCATCCTCCTCTA 3'	179 bps
β-Actin exon 5	−134 to +53	F 5'GCTCTTTTCCAGCCTTCCTT 3' R 5'CGGATGTCAACGTCACACTT 3'	187 bps

Table 2C PCR cloning primers
NRF-1 mutated nucleotide sequences are in boldface.
Cloning Primers	Primer sequence
Nos1	F 5′AAGGTACCGCCAGGTGACCACCACTAAC 3′ R 5′AAAAGCTTCTGACGCATGGCTGGTTTAC 3′
MCOX6b1	F 5'AAGGTACCGCCAGCCCTTAATTGTTTTC3' R 5'AAAAGCTTTCGCAACTAAAAGCTCCACA 3'
Mutagenesis Primers
Nos1Mut	F 5′ GAGGTGCCGCGGATTTGAGCGTTCTTATCCAAGCC 3′ R 5′ GGCTTGGATAAGAACGCTCAAATCCGCGGCACCTC 3′
MCOX6b1Mut	F 5'CAGCACTAGTTAGGCAGAGTTTGGCGGATTTCTGAGTCTAC3' R 5'GTAGACTCAGAAATCCGCCAAACTCTGCCTAACTAGTGCTGG 3'