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Diminished E2k in HEK cells increases the H2O2 induced ROS and cell death. Cells with various levels of E2k protein subunits were utilized. KGDHC activity declined in the cells with about a 50 percent reduction but returned to normal in the lines with the lowest levels of E2k. The cells were incubated with H2O2 and cell death and fluorescence were determined.32
