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. 2009 Aug 27;9:181. doi: 10.1186/1471-2180-9-181

Figure 4.

Figure 4

Bacteriocin activity and transcription analysis of Pectobacterium carotovorum subsp. carotovorum. (A) Transcription analysis of the flhD, flhC, caroS1K, and fliC genes. Total RNA (20 μg) from H-rif-8-6 (WT), TH12-2 (ΔflhC), TH12-2/pBYL2C (ΔflhC/flhC+), KH17 (ΔflhD), KH17/pBYL2D (ΔflhD/flhD+), FliC-KO (ΔfliC), FliC-KO/pBFC (ΔfliC/fliC+), FlhA-KO (ΔflhA), and FlhA-KO/pBFA (ΔflhA/flhA+) cells incubated in BSM medium at 28°C for 24 h was subjected to Northern blot analysis. Strain Ea1068 was used as an indicator for bacteriocin activity. (B) Bacteriocin activity assay. Numbered strains: 1, H-rif-8-6 (wild type); 2, TH12-2 (ΔflhC); 3, KH17 (ΔflhD); 4, TH12-2/pBYL2C (ΔflhC/flhC+); 5, TH12-2/pBYL2DC (ΔflhC/flhDC+); 6, KH17/pBYL2D (ΔflhD/flhD+); 7, KH17/pBYL2DC (ΔflhD/flhDC+); 8, FliC-KO (ΔfliC); 9, FlhA-KO (ΔflhA); 10, FliC-KO/pBFC (ΔfliC/fliC+); and 11, FlhA-KO/pBFA (ΔflhA/flhA+). Strain Ea1068 was used as an indicator for bacteriocin activity.