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. 2009 Aug 27;9:181. doi: 10.1186/1471-2180-9-181

Table 1.

Bacteria and plasmids used in this study

Bacterium or plasmid Relevant characteristics Source
E. coli
 1830 pro-met-KmrNmr, containing transposon Tn5 on the ?sucidal? plasmid pJB4JI Gantotti et al. [37]
 DH5 supE44hsdR17recA1endA1gyrA1thi-1relA1 Hanahan and Reusch et al [26,38]
Pectobacterium carotovorum subsp. carotovorum
 89-H-4 putative biocontrol agent Laboratory stock
 H-rif-8-6 89-H-4, Rifr this work
 Ea1068 wild type Laboratory stock
 T-29 wild type Laboratory stock
 E108 wild type Laboratory stock
 A-100 wild type Laboratory stock
 86-H-2 wild type Laboratory stock
 TH12-2 H-rif-8-2, flhC:: Tn5, Rifr, Kanr this work
 KH17 H-rif-8-2, flh D::Kan, Rifr, Kanr this work
 FliC-KO H-rif-8-2, fli C::Kam, Rifr, Kamr this work
 FlhA-KO H-rif-8-2, flh A::Kam, Rifr, Kamr this work
plasmid
 pBR322 Ampr, Kanr Bolivar et al [39]
 pBYL2DC Ampr, flhDC this work
 pBYL2C Ampr, flhC this work
 pBYL2D Ampr, flhD this work
 pBFC Ampr, fliC this work
 pBFA Ampr, flhA this work

Ampr indicates ampcillin resistance, Rifr indicates rifampicin resistance, and Kanr indicates Kanamycin resistance.