Table 1.
Bacteria and plasmids used in this study
Bacterium or plasmid | Relevant characteristics | Source |
---|---|---|
E. coli | ||
1830 | pro-met-KmrNmr, containing transposon Tn5 on the ?sucidal? plasmid pJB4JI | Gantotti et al. [37] |
DH5 | supE44hsdR17recA1endA1gyrA1thi-1relA1 | Hanahan and Reusch et al [26,38] |
Pectobacterium carotovorum subsp. carotovorum | ||
89-H-4 | putative biocontrol agent | Laboratory stock |
H-rif-8-6 | 89-H-4, Rifr | this work |
Ea1068 | wild type | Laboratory stock |
T-29 | wild type | Laboratory stock |
E108 | wild type | Laboratory stock |
A-100 | wild type | Laboratory stock |
86-H-2 | wild type | Laboratory stock |
TH12-2 | H-rif-8-2, flhC:: Tn5, Rifr, Kanr | this work |
KH17 | H-rif-8-2, flh D::Kan, Rifr, Kanr | this work |
FliC-KO | H-rif-8-2, fli C::Kam, Rifr, Kamr | this work |
FlhA-KO | H-rif-8-2, flh A::Kam, Rifr, Kamr | this work |
plasmid | ||
pBR322 | Ampr, Kanr | Bolivar et al [39] |
pBYL2DC | Ampr, flhDC | this work |
pBYL2C | Ampr, flhC | this work |
pBYL2D | Ampr, flhD | this work |
pBFC | Ampr, fliC | this work |
pBFA | Ampr, flhA | this work |
Ampr indicates ampcillin resistance, Rifr indicates rifampicin resistance, and Kanr indicates Kanamycin resistance.