Table 1.

Gene specific primer sequences and PCR conditions

Gene	Acc. number	Forward primer	Reverse primer	Tm (°C)	Reference
OAS2	AY288913	ACAGTCTTGAGGGGCAACTCTGA	GCTGGCTTTCATCATATCCAAGGA	60
CXC10	AY789646	TGCCCACATGTTGAGATCAT	CGGCCCATCCTTATCAGTAG	60
TRAIL	NM_001024696	CAACAAGGCATTCCTCACCT	CCAGCTCTCCATTCCTCAAG	60
IFN alpha	NM_214393	TACTCAGCTGCAATGCCATC	TCTGTGCTGAAGAGCTGGAA	58
18S RNA	AF102857	GTTCAAAGCAGGCCCGAG	CGCCGCCGCATCGCCA	57	De groot et al. (2005)
Beta actine	AY550069.1	GGCATCCTGACCCTCAAGTA	GGGTCATCTTCTCACGGTTG	58
C4-bp	NM_213942	CTCTGGTTGGAGAGGACAGA	GCTCACAGTCTTCGGGGTA	60

PCR conditions: 3 mM magnesium chloride and 0.5 mM for primer pair. The cycling PCR conditions consisted of 1 cycle at 95°C for 5′, followed by 45 cycles (95°C for 5′, annealing temperature for 5′, 72°C for 12′) and 1 three-segment cycle of product melting