Table 1.
Comparison of immunopathological parameters in L. major infected mice of the strains BALB/c and CcS-11
| Phenotype | Strain | P value | |||
|---|---|---|---|---|---|
| BALB/c | CcS-11 | Strain difference | |||
| Lesion size 5 weeks (mm2) | 60.79 ± 6.33 (n = 19) | 29.55 ± 6.33 (n = 19) | 1.34 × 10−3 | ||
| Lesion size 6 weeks (mm2) | 91.67 ± 7.78 (n = 19) | 41.33 ± 7.78 (n = 19) | 5.88 × 10−5 | ||
| Lesion size 7 weeks (mm2) | 107.42 ± 9.52 (n = 19) | 46.54 ± 9.52 (n = 19) | 6.91 × 10−5 | ||
| Lesion size 8 weeks (mm2) | 129.31 ± 11.73 (n = 19) | 55.05 ± 11.73 (n = 19) | 7.86 × 10−5 | ||
| Splenomegaly (spleen-to-body weight ratio × 1,000) | 17.84 ± 1.13 (n = 19) | 12.26 ± 1.13 (n = 19) | 1.51 × 10−3 | ||
| Hepatomegaly (liver-to-body weight ratio × 1,000) | 68.06 ± 1.55 (n = 19) | 60.32 ± 1.55 (n = 19) | 2.75 × 10−3 | ||
| IgE in serum (μg/ml) | 10.22 | 3.54 ± 0.29 (n = 16) | 3.97 | 1.62 ± 0.28 (n = 18) | 6.79 × 10−5 |
| IFNγ in serum (ng/ml) | 5.57 | 1.31 ± 0.05 (n = 18) | 4.45 | 1.22 ± 0.06 (n = 14) | 0.257 |
| IL-12 in serum (ng/ml) | 2.83 | 1.04 ± 0.20 (n = 16) | 1.74 | 0.55 ± 0.20 (n = 15) | 0.119 |
| IL-4 in serum (ng/ml) | 4.65 | 1.61 ± 0.23 (n = 12) | 2.33 | 0.83 ± 0.26 (n = 9) | 3.75 × 10−2 |
| Parasites in draining lymph nodes, total group (arbitrary units) | 1.61 | 5.08 ± 0.20 (n = 17) | 2.30 | 5.44 ± 0.19 (n = 19) | 0.211 |
| Parasites in draining lymph nodes, females | 0.57 | 4.04 ± 0.23a (n = 9) | 0.46 | 3.83 ± 0.22b (n = 10) | 0.643 |
| Parasites in draining lymph nodes, males | 9.28 | 6.83 ± 0.37a (n = 8) | 8.54 | 6.75 ± 0.35b (n = 9) | 0.931 |
| Parasites in spleen, total group (arbitrary units) | 1.58 | 5.06 ± 0.29 (n = 18) | 0.31 | 3.43 ± 0.28 (n = 19) | 4.02 × 10−4 |
| Parasites in spleen, females | 1.10 | 4.70 ± 0.45c (n = 8) | 0.18 | 2.89 ± 0.40d (n = 10) | 8.36 × 10−3 |
| Parasites in spleen, males | 3.08 | 5.73 ± 0.34c (n = 10) | 0.58 | 4.06 ± 0.36d (n = 9) | 6.01 × 10−3 |
Means and standard errors of means and P values were computed by analysis of variance. Lesion size (mm2), splenomegaly and hepatomegaly (spleen and liver-to-body weight ratio × 1,000, respectively) exhibited normal distribution without transformation. The following transformations were used to obtain normal distribution: IgE levels in serum: IgE (μg/ml)—natural logarithm of value to the power of 1.5; IFNγ (ng/ml)—natural logarithm of the value to the power of 0.5; IL-4 (ng/ml)—natural logarithm to the power of 1.1; IL-12 (ng/ml)—natural logarithm; parasite load detected as parasite DNA content (arbitrary units) in spleen and in lymph nodes—natural logarithm of (value × 100). Number of tested mice is shown in brackets. The numbers in bold give the average non-transformed values.
aSex difference in BALB/c, P = 9.26 × 10−5
bSex difference in CcS-11, P = 1.14 × 10−5
cSex difference in BALB/c, P = 0.133
dSex difference in CcS-11, P = 8.93 × 10−3