Table 3.
Loci that control parasite load in draining lymph nodes and spleens and splenomegaly in L. major infected F2 hybrids between CcS-11 and BALB/c
| Phenotype | Locus | Group | Marker | Genotype | P value | Corrected P value | Percent of explained variance | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CC | CS | SS | ||||||||||
| Parasites in draining lymph nodes (arbitrary units) | Lmr20 | Both sexes | D1Mit403 | 7.74 ± 0.51 (n = 66) | 6.26 ± 0.33 (n = 152) | 4.35 ± 0.51 (n = 66) | 8.35 × 10−5 | 5.54 × 10−3 | 4.25 | |||
| Females | D1Mit403 | 4.96 ± 0.55 (n = 37) | 3.34 ± 0.40 (n = 70) | 1.90 ± 0.55 (n = 37) | 1.16 × 10−3 | 5.89 × 10−2 | 8.39 | |||||
| Males | D1Mit403 | 10.87 ± 0.89 (n = 29) | 9.34 ± 0.53 (n = 82) | 7.31 ± 0.89 (n = 29) | 0.301 | NS | – | |||||
| Parasites in spleen (arbitrary units) | Lmr5 | Both sexes | D10Mit12 | 2.09 | 5.34 ± 0.14 (n = 60) | 0.98 | 4.58 ± 0.09 (n = 150) | 0.96 | 4.56 ± 0.12 (n = 79) | 8.09 × 10−5 | 5.39 × 10−3 | 4.54 |
| Females | D10Mit12 | 1.60 | 5.07 ± 0.20 (n = 31) | 0.60 | 4.10 ± 0.14 (n = 69) | 0.52 | 3.96 ± 0.17 (n = 46) | 8.57 × 10−5 | 5.76 × 10−3 | 11.74 | ||
| Males | D10Mit12 | 2.57 | 5.55 ± 0.20 (n = 29) | 1.70 | 5.14 ± 0.12 (n = 81) | 2.15 | 5.37 ± 0.18 (n = 33) | 0.173 | NS | – | ||
| Splenomegaly (spleen-to-body weight ratio × 1,000) | Lmr5 | Both sexes | D10Mit12 | 21.43 ± 0.61 (n = 62) | 18.82 ± 0.39 (n = 151) | 17.49 ± 0.53 (n = 82) | 8.63 × 10−6 | 6.98 × 10−4 | 11.60 | |||
| D10Mit46 | 20.64 ± 0.63 (n = 61) | 18.76 ± 0.40 (n = 151) | 17.52 ± 0.56 (n = 76) | 1.27 × 10−3 | 6.21 × 10−2 | 4.32 | ||||||
| Females | D10Mit12 | 22.59 ± 0.86 (n = 33) | 19.06 ± 0.59 (n = 70) | 16.91 ± 0.71 (n = 48) | 6.48 × 10−6 | 5.57 × 10−4 | 14.47 | |||||
| D10Mit46 | 22.56 ± 0.96 (n = 28) | 19.42 ± 0.59 (n = 73) | 17.27 ± 0.72 (n = 49) | 1.06 × 10−4 | 7.08 × 10−3 | 11.50 | ||||||
| Males | D10Mit12 | 19.42 ± 0.84 (n = 29) | 18.29 ± 0.50 (n = 81) | 17.71 ± 0.77 (n = 34) | 0.318 | NS | – | |||||
| D10Mit46 | 18.78 ± 0.78 (n = 33) | 18.07 ± 0.51 (n = 78) | 18.34 ± 0.87 (n = 27) | 0.750 | NS | – | ||||||
Mean and SE values were obtained by analysis of variance. Only linkages significant at whole genome level in the total group or in at least in one sex are given. The parasite loads were estimated by PCR–ELISA (see Materials and methods). In order to obtain normal distribution required for analysis of variance, value of parasites in spleens was transformed to the natural logarithm of (value × 100). Splenomegaly (spleen-to-body weight ratio × 1,000) and parasite load in draining lymph nodes exhibited normal distribution without transformation. The numbers in bold give the average non-transformed values. C and S indicate the presence of BALB/c and STS allele, respectively; n indicates number of mice.