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. Author manuscript; available in PMC: 2010 Sep 1.

Published in final edited form as: Exp Parasitol. 2009 Jun 3;123(1):58–64. doi: 10.1016/j.exppara.2009.05.012

Inhibition of apoptosis is species and strain dependent. RAW 264.7 macrophages were infected with L. major strains (V1, Spock, IR173, LV39, and NIH S) (a) or L. donovani strains (1S, 9515, and Mongi) (c) 4 hrs prior to ETOH (■), CHX (), or no additional (□) treatment for 16 hrs. Apoptosis was determined using a flow cytometry based BrdU staining. Mean ± SEM of % apoptosis from 3 independent experiments is presented. *p ≤ 0.05 compared to uninfected CHX treatment unless noted. (b & d) Infections were quantified to ensure equal parasite infection and presented as number of parasites per 100 cells. No statistically significant differences of infection rates were detected.

Inline graphic — Inhibition of apoptosis is species and strain dependent. RAW 264.7 macrophages were infected with L. major strains (V1, Spock, IR173, LV39, and NIH S) (a) or L. donovani strains (1S, 9515, and Mongi) (c) 4 hrs prior to ETOH (■), CHX (), or no additional (□) treatment for 16 hrs. Apoptosis was determined using a flow cytometry based BrdU staining. Mean ± SEM of % apoptosis from 3 independent experiments is presented. *p ≤ 0.05 compared to uninfected CHX treatment unless noted. (b & d) Infections were quantified to ensure equal parasite infection and presented as number of parasites per 100 cells. No statistically significant differences of infection rates were detected.