Expression of neuronal, mesodermal, and early osteogenic markers and integrins after 12 days of culture under osteogenic conditions. (A) PCR on NF matrix (N), solid films (S), and gelatin-coated tissue culture plastic (C). (B) Quantification of brachyury expression on nanofibrous matrix (Nano), solid films (Solid), and gelatin-coated tissue culture plastic (Control); *p < 0.05. (C) Quantification of nestin expression on nanofibrous matrix (Nano), solid films (Solid), and gelatin-coated tissue culture plastic (Control); *p < 0.05.