Fig. 10.
Effect of siRNA knockdown of IL-12Rβ1 and IL-12Rβ2 on the activation of NF-κB and C/EBPβ in p402- and p70-stimulated mouse BV-2 microglial cells. Cells were co-transfected with siRNA constructs against IL-12Rβ1 or IL-12Rβ2 and reporter constructs for NF-κB (A) or C/EBPβ (B). Twenty-four hours of transfection, cells were stimulated with 10 ng/mL p402 and p70 for 6 h followed by analysis of luciferase activities. Data are mean + SD of three different experiments. *P < 0.001 vs. pSilencer-p402; **P < 0.001 vs. pSilencer-p70.