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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Glia. 2009 Nov 1;57(14):1553–1565. doi: 10.1002/glia.20869

Fig. 3.

Fig. 3

Effect of siRNA knockdown of IL-12Rβ1 and IL-12Rβ2 on the expression of iNOS in BV-2 microglial cells. A: Cells were transfected with different amounts of the empty vector (pSilencer) and siRNA against IL-12Rβ1 (si-β1). Forty-eight hours after transfection, cells were analyzed for the expression of IL-12Rβ1 and IL-12Rβ2 by RT-PCR. B: Cells were transfected with the empty vector and siRNA against IL-12Rβ2 (si-β2). Forty-eight hours after transfection, cells were analyzed for the expression of IL-12Rβ1 and IL-12Rβ2. Twenty-four hours after transfection, cells were stimulated with 10 ng/mL p402 and p70 under serum-free condition. After 6 h of stimulation, the expression of iNOS mRNA was examined by RT-PCR (C) and quantitative real-time PCR (D). After 24 h of stimulation, concentration of nitrite (E) was measured in supernatants. Data are mean + SD of three separate experiments.