Fig. 7.

Role of IL-12Rβ1 and IL-12Rβ2 on the activation of Erk and p38 MAP kinases in p40₂-stimulated mouse microglia. Mouse BV-2 microglial cells were transfected with the empty vector (pSilencer) and siRNA against IL-12Rβ1 (si-β1) and IL-12Rβ2 (si-β2). Twenty-four hours after transfection, cells were stimulated with 10 ng/mL p40₂ under serum-free condition. At different minute intervals, activities of Erk (A) and p38 (B) MAP kinases were assayed by immuno-complex kinase assay. Primary microglia isolated from wild type, IL-12Rβ1 (−/−) and IL-12Rβ2 (−/−) mice were stimulated with 10 ng/mL p40₂. At different minute intervals, activation status of ERK (C) and p38 (D) was monitored by western blot using antibodies against either phospho-MAP kinases or total MAP kinases. Results represent three independent experiments.