TABLE 1.
Effect of SB203580 and PD98059 on p402-Induced Production of Nitrite in Primary Mouse Microglia
| Nitrite (nmol/mg protein/24 h) | |||||
|---|---|---|---|---|---|
| Control | p402 | p402 + SB (20 μM) | p402 + SB (30 μM) | p402 + PD (25 μM) | p402 + PD (50 μM) |
| 3.4 ± 0.4 | 19.6 ± 2.3* | 9.1 ± 0.6** | 4.5 ± 0.8** | 12.5 ± 0.9*** | 7.8 ± 1.2** |
Primary microglia preincubated with different concentrations of SB203580 and PD98059 for 30 min were stimulated with 10 ng/mL p402. After 24 h, supernatants were used for nitrite assay. Data are mean ± S.D. of three different experiments.
**
P < 0.001 vs. control;
**
P < 0.001 vs. p402;
***
P < 0.05 vs. p402.