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. Author manuscript; available in PMC: 2010 May 14.
Published in final edited form as: Mol Cell. 2009 May 14;34(4):497–509. doi: 10.1016/j.molcel.2009.04.011

Figure 6. 14-3-3 regulates the apoptotic activity of PACS-2 Ser437.

Figure 6

(A) HCT116 cells expressing HA-tagged PACS-2 and myc-tagged 14-3-3ζ were treated with 25 ng/ml TRAIL for the indicated times. PACS-2ha immunoprecipitates were immunoblotted to detect co-precipitating 14-3-3ζ and pSer437. (B) TAg-PACS-2-/- cells were co-transfected with an eYFP-tagged caspase-3 reporter together with pcDNA3.1 (vector) or plasmids expressing HA-tagged PACS-2 or PACS-2S437A alone or with 14-3-3ζ-myc. Top: Cells treated with 10 ng/ml mouse TRAIL for 16 hr were fixed and the number of nuclear eYFP-positive cells indicating activated caspase-3 were analyzed. Data are represented as mean +/- SEM. Bottom: Representative immunofluorescence images of the quantified data. Insets, co-expression of 14-3-3ζ-myc and HA-tagged PACS-2 or PACS-2S437A. Scale bar, 20 μm. (C) TAg-PACS-2-/- cells described in B were lysed, PACS-2 immunoprecipitated and co-precipitating 14-3-3ζ-myc detected by western blot.