Table 1.
Antibodies used and technical details
| Antibodies to | Source | Clone | Dilution | Epitope retrieval |
|---|---|---|---|---|
| CD45RB | DAKO | 2B11+PD7 | 1:500 | MW-CB |
| CD30 | DAKO | Ber-H2 | 1:300 | MW-CB |
| p63 | Lab Vision | 4A4 | 1:300 | PC-CB |
| CD20 | DAKO | L26 | 1:1200 | MW-CB |
| CD3 | Lab Vision | SP7 | 1:200 | S-CB |
| ALK | DAKO | ALK1 | 1:200 | S-TRIS |
| CD25 | Novocastra | 4C9 | 1:200 | S-EDTA |
| CD15 | B-Dickinson | Leu-M1 | 1:50 | MW-CB |
| EMA | DAKO | E29 | 1:800 | PC-CB |
| Ki-67 | DAKO | MIB-1 | 1:4800 | PC-CB |
| CD7 | Novocastra | 272 | 1:400 | PC-CB |
| CD2 | Novocastra | 11F11 | 1:200 | MW-CB |
| CD4 | Novocastra | 4B12 | 1:100 | S-EDTA |
| CD5 | Novocastra | 4C7 | 1:150 | MW-CB |
| Clusterin | Novocastra | 7D1 | 1:50 | MW-CB |
| PAX-5 | B-Dickinson | 24 | 1:100 | S-CB |
NOTE. DAKO, Carpinteria, CA; Lab Vision Corporation, Fremont, CA, Novocastra, Newcastle Upon Tyne, United Kingdom; B-Dickinson: Becton-Dickinson Biosciences, Mountain View, CA. Heat-induced epitope retrieval was used.
Abbreviations: EMA, epithelial membrane antigen; MW, microwave oven; PC, pressure cooker; S, steamer; CB, citrate buffer.