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. 2009 Sep 24;4(9):e7153. doi: 10.1371/journal.pone.0007153

Figure 4. Glu-induced GABA release is not mediated by vesicular release.

Figure 4

(A) Elevation of [GABA]o in acute rat hippocampal slices in control conditions (n = 6) and in the presence of glutamate receptor antagonists (n = 7; P = 0.014; 128.5±13.4% of pre-stimulus control). (B) Cytosolic GABA level as determined by [3H]GABA uptake in the presence of 100 µM NNC-711 and Glu receptor antagonists in rat cerebrocortical NPMV fractions (n = 4; P = 0.82) (C) Elevation of [GABA]o in acute rat hippocampal slices in control conditions (n = 6) and in low-[Ca2+]/high-[Mg2+] buffer (n = 5; P = 0.91; 148.3±15.5% of pre-stimulus control). (D) Cytosolic GABA level as determined by [3H]GABA uptake in the presence of 100 µM NNC-711 in low-[Ca2+] buffer in rat cerebrocortical NPMV fractions (n = 3; P = 0.49). Low-[Ca2+] buffer contained 145 mM NaCl, 5 mM KCl, 20 mM MgCl2, 10 mM glucose and 20 mM HEPES (pH 7.5).