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. 2009 Sep 24;4(9):e7153. doi: 10.1371/journal.pone.0007153

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Héja et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Contour plot representations of gradient enhanced ¹H/¹³C heteronuclear single quantum coherence (¹³C-HSQC) spectra of extracellular fluid sample from rat cerebrocortical NPMV after incubation with [U-¹³C/¹⁵N]Glu for 30 min in control (black) and semicarbazide (SCB)-treated animal (red). GABA resonances (arrows) do not appear in the samples from SCB-treated animal. (B) Elevation of [GABA]_o in acute rat hippocampal slices in control (n = 6) and SCB-treated animals (n = 7; P = 0.54; 145±14% of pre-stimulus control). (C) Intracellular [³H]GABA level as determined by [³H]GABA uptake in the presence of 100 µM NNC-711 in control condition and in SCB-treated animals (n = 3, P = 0.85).