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. 2009 Sep 24;4(9):e7153. doi: 10.1371/journal.pone.0007153

Figure 7. Glu-induced GABA release requires the concerted action of Glu and GABA transporters.

Figure 7

(A) Elevation of [GABA]o in acute rat hippocampal slices in control conditions (n = 6) and in the presence of the specific GAT-2/3 blocker, SNAP-5114 (n = 4, P<0.001; 109±4% of pre-stimulus control). (B) Cytosolic β-alanine level as determined by [3H]β-alanine uptake in rat cerebrocortical NPMV fraction (n = 3). (C) Elevation of [GABA]o in acute rat hippocampal slices in control conditions (n = 6) and in the presence of the Glu transporter blocker, TBOA (100 µM: n = 4, P<0.001; 123±10% of pre-stimulus control, 1000 µM: n = 4, P<0.001; 109±1% of pre-stimulus control). (D) Cytosolic GABA level as determined by [3H]GABA uptake in the presence of 100 µM NNC-711 and Glu transporter blockers in rat cerebrocortical NPMV fraction (n = 3; P<0.001 for both DHK and TBOA). (E) Alteration of [GABA]o in acute rat hippocampal slices in normal ACSF (control, n = 5, 100±6% of pre-application control) and in nominally Mg2+-free ACSF (low-[Mg2+], n = 5, P<0.001; 90±4% of pre-application control) during blockade of GAT-2/3 by the specific, non-transportable inhibitor SNAP-5114.